三道湾水电站正常溢洪洞及泄洪排

试验表明，三道湾水电站原设计存在以下问题：泄洪排沙洞单独全开运行工况下，其进口上方存在贯通性吸气漏斗漩涡；正常溢洪洞进口侧收缩十分严重，泄流量不足，闸后存在水翅、折冲波等不良流态，下游消能效果不佳；针对实际情况，试验通过在泄洪排沙洞进口增设消涡格栅，在正常溢洪洞进口设置椭圆形导墙、延长闸后收缩段，下游采用扩散挑流消能等措施，很好地解决了上述问题。     

Yield performance of spring wheat improved by regulated deficit irrigation in an arid area

A field experiment was conducted in 2003 and 2004 growing seasons to evaluate the effects of regulated deficit irrigation on yield performance in spring wheat (Triticum aestivum) in an arid area. Three regulated deficit irrigation treatments designed to subject the crops to various degrees of soil water deficit at different stages of crop development and a no-soil-water-deficit control was established. Soil moisture was measured gravimetrically in the increment of 0–20 cm every five to seven days in the given growth periods, while that in 20 increments to 40, 40–60, 60–80, and 80–100 cm depth measured by neutron probe. Compared to the no-soil-water-deficit treatment, grain yield, biomass, harvest index, water use efficiency (WUE), and water supply use efficiency (WsUE) in spring wheat were all greatly improved by 16.6–25.0, 12.4–19.2, 23.5–27.3, 32.7–39.9, and 44.6–58.8% under regulated deficit irrigation, and better yield components such as thousand-grain weight, grain weight per spike, number of grain, length of spike, and fertile spikelet number were also obtained, but irrigation water was substantially decreased by 14.0–22.9%. The patterns of soil moisture were similar in the regulated deficit treatments, and the soil moisture contents were greatly decreased by regulated deficit irrigation during wheat growing seasons. Significant differences were found between the no-soil-water-deficit treatment and the regulated soil water deficit treatments in grain yield, yield components, biomass, harvest index, WUE, and WsUE, but no significant differences occurred within the regulated soil water deficit treatments. Yield performance proved that regulated deficit irrigation treatment subjected to medium soil water deficit both during the middle vegetative stage (jointing) and the late reproductive stages (filling and maturity or filling) while subjected to no-soil-water-deficit both during the late vegetative stage (booting) and the early reproductive stage (heading) (MNNM) had the highest yield increase of 25.0 and 14.0% of significant water-saving, therefore, the optimum controlled soil water deficit levels in this study should range 50–60% of field water capacity (FWC) at the middle vegetative growth period (jointing), and 65–70% of FWC at both of the late vegetative period (booting) and early reproductive period (heading) followed by 50–60% of FWC at the late reproductive periods (the end of filling or filling and maturity) in treatment MNNM, with the corresponding optimum total irrigation water of 338 mm. In addition, the relationships among grain yield, biomass, and harvest index, the relationship between grain yield and WUE, WsUE, and the relationship between harvest index and WUE, WsUE under regulated deficit irrigation were also estimated through linear or non-linear regression models, which indicate that the highest grain yield was associated with the maximum biomass, harvest index, and water supply use efficiency, but not with the highest water use efficiency, which was reached by appropriate controlling soil moisture content and water consumption. The relations also indicate that the harvest index was associated with the maximum biomass and water supply use efficiency, but not with the highest water use efficiency.     

Whole-genome re-sequencing association study on yearling wool traits in Chinese fine-wool sheep

To investigate single nucleotide polymorphism (SNP) loci associated with yearling wool traits of fine-wool sheep for optimizing marker-assisted selection and dissection of the genetic architecture of wool traits, we conducted a genome-wide association study (GWAS) based on the fixed and random model circulating probability unification (FarmCPU) for yearling staple length (YSL), yearling mean fiber diameter (YFD), yearling greasy fleece weight (YGFW), and yearling clean fleece rate (YCFR) by using the whole-genome re-sequenced data (totaling 577 sheep) from the following four fine-wool sheep breeds in China: Alpine Merino sheep (AMS), Chinese Merino sheep (CMS), Qinghai fine-wool sheep (QHS), and Aohan fine-wool sheep (AHS). A total of 16 SNPs were detected above the genome-wise significant threshold (P = 5.45E-09), and 79 SNPs were located above the suggestive significance threshold (P = 5.00E-07) from the GWAS results. For YFD and YGFW traits, 7 and 9 SNPs reached the genome-wise significance thresholds, whereas 10 and 12 SNPs reached the suggestive significance threshold, respectively. For YSL and YCFR traits, none of the SNPs reached the genome-wise significance thresholds, whereas 57 SNPs exceeded the suggestive significance threshold. We recorded 14 genes located at the region of ±50-kb near the genome-wise significant SNPs and 59 genes located at the region of ±50-kb near the suggestive significant SNPs. Meanwhile, we used the Average Information Restricted Maximum likelihood algorithm (AI-REML) in the “HIBLUP” package to estimate the heritability and variance components of the four desired yearling wool traits. The estimated heritability values (h²) of YSL, YFD, YGFW, and YCFR were 0.6208, 0.7460, 0.6758, and 0.5559, respectively. We noted that the genetic parameters in this study can be used for fine-wool sheep breeding. The newly detected significant SNPs and the newly identified candidate genes in this study would enhance our understanding of yearling wool formation, and significant SNPs can be applied to genome selection in fine-wool sheep breeding.     

不同生物质炭输入水平下旱作农田温室气体排放研究

在陇中黄土高原干旱半干旱区,采用小区定位试验,对不同生物质炭输入水平下春小麦农田土壤温室气体(CO_2、N_2O和CH_4)的排放通量进行全生育期连续观测,并分析其影响因子。结果表明:6个生物质炭输入水平处理下[0 t·hm~(-2)(CK)、10 t·hm~(-2)、20 t·hm~(-2)、30 t·hm~(-2)、40 t·hm~(-2)、50 t·hm~(-2)],旱作农田土壤在春小麦全生育期内均表现为CH_4弱源、N_2O源和CO_2源。全生育期各处理CH_4平均排放通量依次为:0.005 7 mg·m~(-2)·h~(-1)、0.0047 mg·m~(-2)·h~(-1)、0.003 6 mg·m~(-2)·h~(-1)、0.003 3 mg·m~(-2)·h~(-1)、0.002 7 mg·m~(-2)·h~(-1)和0.000 4 mg·m~(-2)·h~(-1),N_2O平均排放通量依次为:0.230 5 mg·m~(-2)·h~(-1)、0.144 1 mg·m~(-2)·h~(-1)、0.135 3 mg·m~(-2)·h~(-1)、0.098 9 mg·m~(-2)·h~(-1)、0.125 0 mg·m~(-2)·h~(-1)和0.151 3mg·m~(-2)·h~(-1),CO_2平均排放通量依次为:0.449 2μmol·m~(-2)·s~(-1)、0.447 0μmol·m~(-2)·s~(-1)、0.430 3μmol·m~(-2)·s~(-1)、0.391 4μmol·m~(-2)·s~(-1)、0.408 0μmol·m~(-2)·s~(-1)和0.416 4μmol·m~(-2)·s~(-1)。土壤CH_4排放通量随生物质炭输入量的增加而减小;当生物质炭输入量小于30 t·hm~(-2)时,土壤N_2O、CO_2排放通量随其输入量增加而显著减小,但当其输入量超过30 t·hm~(-2)时,N_2O、CO_2排放通量则呈显著增大趋势。各处理在5~15 cm土层平均土壤温度差异显著(P0.05),在5~10 cm土层平均土壤含水量差异显著(P0.05),土壤温度及含水量受生物质炭影响明显;且CK处理不同土层的土壤温度及含水量波动最大,生物质炭输入可在一定程度上降低不同土层土壤的水热变化幅度;N_2O、CO_2排放通量与10~15 cm土层土壤温度呈显著性负相关,与20~25 cm土壤温度呈显著性正相关;CH_4平均排放通量与5~10 cm土层土壤温度呈显著性负相关,与其含水量呈显著性正相关;N_2O平均排放通量与15~20 cm土层土壤温度呈显著性正相关;CH_4、N_2O、CO_2平均排放通量与0~5 cm土层土壤水分呈显著性负相关。生物质炭的输入能够减小温室气体的排放,且会因其输入量的不同而异,因此适量应用生物质炭有利于旱作农田生育期内增汇减排。     

陇东耕地净第一性生产力及生态服务价值的时空分异研究

为促进甘肃陇东耕地生态系统和经济持续健康发展,该研究采用生产力和生态服务价值的估算方法,对研究区域耕地净第一性生产力(NPP)和生态服务功能的时空分异进行定量分析。结果表明,1997－2005年陇东黄土高原耕地的NPP和生态服务单位面积价值变化不明显,而总量略有上升。同时,耕地的NPP和生态服务单位面积价值在空间分布上有显著的区域差异性,该区域可分成NPP和生态服务价值相对较低的西北区和相对较高的东南区。在西北区,耕地生态服务单位面积价值为7.7万元/(hm²·a),NPP只有3.54 t/(hm²·a);在东南区,生态服务单位面积价值为10.2万元/(hm²·a),NPP为5.94 t/(hm²·a)。因此,要通过合理调整种植结构和作物布局,充分利用降水资源,以提高研究区耕地的NPP和生态服务价值。     

北方白菜型冬油菜的膜脂脂肪酸组分和ATPase活性对温度的响应

为了解北方白菜型冬油菜膜脂脂肪酸和ATPase活性与抗寒性的关系,以抗寒性强的冬油菜品种陇油7号和抗寒性弱的品种天油2号为材料,研究了不同温度处理(25°C、10°C、2°C、–5°C)后叶片和根系膜脂脂肪酸组分和ATPase酶活性的变化。结果表明,低温胁迫下2个冬油菜品种叶片和根系膜脂脂肪酸组分相同,叶片中不饱和脂肪酸以亚麻酸为主,根系不饱和脂肪酸以亚油酸为主。随处理温度的降低,2个冬油菜品种叶片不饱和脂肪酸含量呈先降低(10°C,2°C)后增加(–5°C)的趋势;陇油7号根中不饱和脂肪酸含量逐步增加,天油2号则逐步降低;在低温条件下(2°C,–5°C),陇油7号膜脂U/S比值、IUFA值高于天油2号;ATPase活性表现为陇油7号逐渐高于天油2号。说明2个冬油菜品种的膜脂在低温响应上存在一定差异,低温下不饱和脂肪酸含量和ATPase活性的提高是强抗寒冬油菜品种在北方旱寒区严酷环境条件下能安全越冬的重要原因。     

不同轮作制度对定西地区农田杂草群落的影响

为探讨不同轮作制度对定西地区农田杂草群落的影响,采用倒置"W"九点取样法,调查了定西地区7种不同轮作制度下田间杂草的种类、数量、地上生物量等。在试验田发现11个科共15种杂草;从杂草发生密度、地上生物量上看,苦苣菜、藜是农田优势杂草,防除的目的杂草;不同轮作田的杂草群落由优势杂草组成;从不同轮作田杂草群落的物种多样性来看,马铃薯胡麻轮作马铃薯小麦轮作胡麻小麦轮作胡麻连作小麦胡麻轮作小麦马铃薯轮作胡麻马铃薯轮作;对不同轮作田杂草群落进行聚类,可分为四类。通过对不同轮作田中杂草的密度、地上生物量和综合优势度比的综合分析,可以看出胡麻连作、小麦胡麻轮作、马铃薯胡麻轮作、小麦马铃薯轮作中杂草的危害性较大。马铃薯小麦轮作、胡麻小麦轮作和胡麻马铃薯轮作对杂草有一定的控制作用。     

基于改进杂交粒子群算法的农村微能网多能流优化调度

西部农村地区电网薄弱,光伏和风电扶贫投资未考虑配套输配电设施,用以处理生物质废弃物的沼气受季节性温度变化影响运行经济性不佳,为解决上述问题,该文提出利用沼气作为气源含可再生能源的冷-热-电-气多能流农村微能网供能架构,建立相应的多能流微能网调度模型,针对粒子群算法早熟、容易陷入局部最优的问题,提出采用动态调整惯性权重的杂交粒子群算法进行求解,算例结果表明,通过对系统内各设备的调度,有效降低系统日运行成本,在冬季,采用改进型杂交粒子群算法所得日运行费用相比采用基本型粒子群算法降低7.6%,其相比系统未优化所得日运行费用降低79.1%;在夏季,相比基本型粒子群算法与未优化分别降低17.0%、71.2%,实现微能网的经济运行,证明了本模型和算法的正确性。     

Trichoderma harzianum T-E5 significantly affects cucumber root exudates and fungal community in the cucumber rhizosphere

The Fusarium wilt in cucumbers, caused by the pathogenic fungus Fusarium oxysporum f. sp. cucumerinum, is a serious and destructive disease worldwide. An effort was made to explore the role of Trichoderma harzianum T-E5 in reducing the incidence of Fusarium wilt. Three treatments (Control, T1, and T2) were established in the greenhouse experiment. The effects of T-E5 on the composition of root exudates and fungal community in the cucumber rhizosphere were measured. Compared with the control, the application of a bio-organic fertilizer (BIO) enriched with T-E5 was found to decrease the incidence of Fusarium wilt notably and promote the growth of cucumber plants. Based on real-time PCR, the population of F. oxysporum in the control without T-E5 increased from 10³ to 10⁴ ITS copies g⁻¹ soil, whereas the population decreased from 10³ to 10² ITS copies g⁻¹ soil in the T1 and T2 treatment groups when T-E5 was included. Significant difference in fungal community was also found among the treatment groups. HPLC analysis showed that the detected levels of phenolic compounds in control were significantly higher than the levels in the samples subjected to T1 and T2 treatments. The root exudates from the control group significantly increased the numbers of germinating spores of the pathogen compared with those from the samples treated with T1 and T2. In conclusion, the modification of root exudates and the fungal community by the application of BIO might account for the effective suppression of Fusarium wilt disease in cucumbers.     

Quantifying the Dynamics of Livestock Distribution by Unmanned Aerial Vehicles (UAVs): A Case Study of Yak Grazing at the Household Scale

Managed grazing is the most extensive land use worldwide. Information about the spatio-temporal distribution of livestock is critical for grassland ecosystem management. However, no direct and cost-effective method exists to monitor livestock distribution under natural conditions. We proposed a practical method that uses unmanned aerial vehicles (UAVs), and tested it at a typical household pasture on the Qinghai-Tibetan Plateau. We monitored and analyzed the hourly spatial distribution of each yak (Bos mutus). We found that (1) yak information extracted by our independently developed software was sufficiently accurate (P > 0.05); (2) yak density (frequency/area/period) decreased with increasing distance from campsites in the early growing season, while the pattern reversed in the late growing season; (3) dispersion index (DI, m²/yak) of the yak herd exhibited a tendency to decrease in the morning and then to increase in the afternoon within a day; from June to September DI decreased in both the morning and afternoon, but was similar at noon. The monitoring method we developed in this study characterized the spatial distribution of the whole yak herd dynamically with high frequency, resolution, and efficiency. We conclude that this method is suitable for long-term monitoring of animal behavior, and for studying relationship between the distribution of pastoral livestock and resource availability, which will be beneficial for grassland sustainable management.